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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in many tumors, which leads to chromosome instability, and thus may well add to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important purpose in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two additional splice variants have been claimed but in depth nucleotide sequence info will not be still obtainable.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide usually includes the epitope acknowledged by the antibody.
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This information outlines further doable optimization of cellular and immunoassay parameters to be sure the very best effects are...
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This tutorial outlines even further possible optimization of cellular and immunoassay parameters to be certain the absolute best success are...
Antibodies bound to the blocking peptide not bind into the epitope around the concentrate on protein. By comparing the staining with the blocked antibody versus the antibody alone, you can see which staining is certain.
Goal Facts Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which add for the temporal coordination of each mitotic celebration. Cyclin E kinds a posh with and features as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is degraded as cells development by means of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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